anti-Cas9 Monoclonal Antibody

Cas9

CRISPR-associated endonuclease Cas9/Csn1, CRISPR associated protein 9, SpyCas9

Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspersed Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes and other bacteria. Cas9 is used to interrogate and cleave foreign DNA, such as invading bacteriophage DNA or plasmid DNA. This is accomplished by unwinding foreign DNA and checking for if it is complementary to the 20 base pair spacer region of the guide RNA. If the DNA substrate is complementary to the guide RNA, Cas9 cleaves the invading DNA.

Mouse

Monoclonal

Mouse IgG1κ

7A9

N-terminal fragment of Streptococcus pyogenes Cas9.

Liquid

PBS containing 0.02% sodium azide.

Affinity purified using protein G

IF, IP, WB

Immunofluorescence: 1:500-1:2,000; Immunoprecipitation: 1:20; Western Blot: 1:1,000

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

anticorps