anti-PARP Antibody (Clone 2B6)

Catalog number
A1109-100
Name
anti-PARP Antibody (Clone 2B6)
Size
PARP Antibody (Clone 2B6)
Price
360.00 EUR
Supplier

Details

Target of this antibody:
PARP
Alternative names of antibody target:
PARP1, ADPRT, PPOL, NAD(+) ADP-ribosyltransferase 1
Background information:
PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like Caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis.
Antibody host:
Mouse
Clonality of antibody:
Monoclonal
Isotype:
Mouse IgG1κ
Clone:
2B6
This antibody reacts with:
Human
Immunogene sequence:
A synthetic peptide corresponding to residues before the cleavage site of human PARP-1 was used as immunogen. This antibody recognizes full-length form of PARP and PARP-1 only.
Concentration of antibody:
1 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
Supplied in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA
Purification method:
Protein G affinity chromatography
This antibody has been succesfully tested for use in following methods:
WB
Recommended usage:
WB: 2-5 µg
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps