anti-Anti-Histone H3 pan, Rabbit Monoclonal Antibody

Catalog number
A1141-25
Name
anti-Anti-Histone H3 pan, Rabbit Monoclonal Antibody
Size
Anti-Histone H3 pan, Rabbit Monoclonal Antibody
Price
262.00 EUR
Supplier

Details

Target of this antibody:
H3F3A
Alternative names of antibody target:
H3F3A, H3.3A, H3F3, H3F3B, H3.3B
Background information:
Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases.
Antibody host:
Rabbit
Clonality of antibody:
Monoclonal
Isotype:
Rabbit IgG
Clone:
RM188
This antibody reacts with:
Human
Immunogene sequence:
A peptide corresponding to the C-terminus of human Histone H3
Concentration of antibody:
1 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
In 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Purification method:
Affinity purified using protein A
This antibody has been succesfully tested for use in following methods:
WB, ELISA, Multiplex, ICC
Recommended usage:
Western Blot: 0.01 µg/ml – 0.25 µg/ml; ICC: 1 µg/ml - 5 µg/ml; ELISA: 0.2 µg/ml - 1 µg/ml; Multiplex: 0.1 µg/ml – 0.5 µg/ml
Storage information:
Shelf time: 13 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rabbits are used for polyclonal antibody production by Biovision. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biovision adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name
Oryctolagus cuniculus
French translation
anticorps