anti-Phospho-AMPKα1 (Ser496) Antibody

PRKAA1

PRKAA1; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1

Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2.

Rabbit

Polyclonal

Rabbit IgG

N/A

Human, mouse , rat

Synthesized peptide derived from human AMPKα1 around the phosphorylation site of S496 (420-500aa)

Liquid

In PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide

Affinity-chromatography using epitope-specific immunogen

WB, ELISA, IHC

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps