Human CellExp™ Progranulin, Rat Recombinant

Progranulin (PGRN), also called proepithelin and PC cell-derived growth factor, is a single precursor protein of granulins which are a family of secreted, glycosylated peptides. It is a widely expressed pluripotent growth factor which plays a role in processes such as development, wound repair and inflammation by activating signaling cascades that control cell cycle progression and cell motility. Its function in the central nervous system is of interest, as mutations in the PGRN gene were found in cases of fronto-temporal degeneration (FTLD). In addition, PGRN has also been linked to tumorigenesis. Progranulin is a biomarker for FTLD, other types of Alzheimer‘s Disease (AD) and potentially for MCI (Mild Cognitive Impairment). Additionally, PGRN is described as a new ligand of TNF receptors and a potential therapeutic against inflammatory disease like arthritis. Rat Recombinant Progranulin fused to FLAG® at C-terminus produced in HEK is a single, glycosylated, polypeptide chain purified by standard chromatographic techniques, containing 602 amino acids and having a molecular mass of ~65.0 kDa.

0.3 mg/ml

~65.0 kDa

Proepithelin; PEPI; PC Cell-derived Growth Factor

Proepithelin; PEPI; PC Cell-derived Growth Factor

10116

GRN

Rat

P23785

Yes

HEK 293 cells

≥95%

SDS-PAGE, HPLC

N/A

N/A

N/A

N/A

N/A

-20°C

Gel pack

Lyophilized protein

Lyophilized from 0.2 µm-filtered solution in PBS.

Reconstitute in dH2O before use.

Signal peptide and rat Progranulin (aa 1-602) fused at the C-terminus to a FLAG®-tag.

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Rattus norvegicus

Recombinants or rec. proteins

recombinants