Human CellExp™ LEFTY-I, Human Recombinant

Catalog number

4873-50

Size

50 μg

Price

1409.00 EUR

Supplier

Biovision

Order

Details

Background

BioVision’s LEFTY-1 is expressed in human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 38 kDa. This cytokine is produced in a human cell expression system with serum-free, chemically defined media. Production in human 293 cells offers authentic glycosylation, contributing to stability in cell growth media. Lefty proteins function as an antagonist of the Nodal Signaling pathway. Proper functioning of Lefty is crucial to the proper development of the heart, lungs, spleen, and liver.

Concentration

N/A

Molecular weight

38 kDa

Synonym

Human Recombinant LEFTY-I

Other name

Left-right determination factor 1, Left-right determination factor B, Protein lefty-1, Protein lefty-B

NCBI gene number

10637

NCBI gene

LEFTY1

Gene source

Human

NCBI number

O75610

Recombinant

Yes

Source

Human 293 cells

Purification

≥90%

Tested applications

SDS-PAGE, HPLC

Tested activity

N/A

Biological activity

The specific activity was determined by dose dependent ability to inhibit BMP-4 (6.5 ng/mL) induction of alkaline phosphatase production in the MC-3T3-E1 cell line (Mouse Chondrogenic cell line).

Reesults

Typically 10 to 40 ng/mL ED50.

Binding ability

N/A

Unit

N/A

Storage condition

-20°C

Shipping under

Blue ice

Physical appearance

Lyophilized protein

Physical properties

Lyophilized in 10 mM Tris-HCl, pH 7.4, 50 mM NaCl, 0.5% CHAPS.

How to reconstitute

Reconstitute in sterile water.

Aa sequence

N/A

Before use

Centrifuge the vial prior to opening.

Notes

For Research Use Only! Not to be used in humans

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Additional source

Recombinants or rec. proteins

Group

recombinants