Caspase-3 Substrate DEVD-AFC for cell activation

Catalog number
1007-1000
Name
Caspase-3 Substrate DEVD-AFC for cell activation
Size
1000 assays
Price
643.00 EUR
Supplier

Details

Description
Ready-to-use fluorometric substrate for caspase-3/CPP32 (Km = 9.7 µM) and related caspases that recognize the amino acid sequence DEVD. The sequence DEVD is based on caspase-3 cleavage site in poly (ADP-ribose) polymerase (PARP). CPP32 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate DEVD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or a multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.
Peptide sequence
Ac-Asp-Glu-Val-Asp-AFC
CAS number
N/A
Molecular weight
729
Category
Caspase Substrate
Other name
Ac-DEVD-AFC, Caspase-3 Substrate Fluorogenic
Molecular formula
C₃₀H₃₃F₃N₅O₁₃
Physical appearance
Liquid
Supplied with
In DMSO (1 mM)
Is this a salt?
No
Is it cell-permeable?
No
Purification
>98% by HPLC
Reconstitute instructions
N/A
Storage condition
-20°C
Shipping condition
gel pack
Maximum time for storage
6-12 months
Storage instructions
Potect from light and moisture
Tissue
cell
Additional description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.For cells, cell lines and tissues in culture till half confluency.