Caspase-8 Substrate IETD-AFC for cell activation

Ready-to-use fluorometric substrate for FLICE/caspase-8 and related caspases that recognize the amino acid sequence IETD. The sequence IETD is based on caspase-8 cleavage site in CPP32/caspase-3 proenzyme. FLICE and related caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate IETD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.

Ac-Ile-Glu-Thr-Asp-AFC

N/A

729.7

Caspase Substrate

Ac-IETD-AFC, Caspase-8 Substrate, Fluorogenic

C₃₁H₃₈F₃N₅O₁₂

Liquid

DMSO (2 mM)

No

No

>95% by HPLC

N/A

-20°C

gel pack

12 months

Protect from light and moisture

cell

Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.For cells, cell lines and tissues in culture till half confluency.