Caspase-8 Substrate IETD-pNA for cell activation
Catalog number
1063-200
Name
Caspase-8 Substrate IETD-pNA for cell activation
Size
200 assays
Price
327.00 EUR
Supplier
Details
Description
Ready-to-use colorimetric substrate for FLICE/caspase-8 and related caspases that recognize the amino acid sequence IETD. The sequence IETD is based on caspase-8 cleavage site in CPP32/Caspase-3 proenzyme. FLICE and related caspase activity can be quantified by spectrophotometric detection of free pNA (l= 400 nm) after cleavage from the peptide substrate IETD-pNA, using a spectrophotometer or multi-well plate reader.
Peptide sequence
Ac-Ile-Glu-Thr-Asp-pNA
CAS number
N/A
Molecular weight
638.6
Category
Caspase Substrate
Other name
Ac-IETD-pNA, Caspase-8 Substrate, Colorimetric
Molecular formula
C₂₇H₃₈N₆O₁₂
Physical appearance
Liquid
Supplied with
DMSO (4 mM)
Is this a salt?
No
Is it cell-permeable?
No
Purification
>95% by HPLC
Reconstitute instructions
N/A
Storage condition
-20°C
Shipping condition
gel pack
Maximum time for storage
12 months
Storage instructions
Protect from light and moisture
Tissue
cell
Additional description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.For cells, cell lines and tissues in culture till half confluency.
