Caspase-9 Substrate LEHD-AFC for cell activation

Ready-to-use fluorometric substrate for caspase-9/Mch-6 and related caspases that recognize the amino acid sequence LEHD. Caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate LEHD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized, using a hand-held long-UV lamp. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large amount of caspase assays. Cell Lysis Buffer (Cat. #1067-100, -400) and 2X Reaction Buffer (Cat. #1068-20, -80) and DTT (Cat.# 1201-1) for caspase assays are also available separately.

Ac-Leu-Glu-His-Asp-AFC

N/A

765.7

Caspase Substrate

Ac-LEHD-AFC, Caspase-9 Substrate, Fluorogenic

C₃₃H₃₈F₃N₇O₁₁

Liquid

DMSO ( 1 mM)

No

No

>98% by HPLC

N/A

-20°C

gel pack

12 months

Protect from light and moisture

cell

Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.For cells, cell lines and tissues in culture till half confluency.