JC-1 for cell activation

JC-1 is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. JC-1 monomer is in equilibrium with so called J-aggregates, which are favored at higher mitochondrial membrane potential. The monomer JC-1 has green fluorescence (lem = 527 nm), while the J-aggregates have red fluorescence (lem = 590 nm). Therefore, it has been possible to use fluorescence ratioing technique to study mitochondrial membrane potential. JC-1 is particularly useful for apoptosis studies. In apoptotic cells, the dye stays in the cytoplasm and fluoresces green, while in healthy cells, the dye aggregates in the mitochondria and fluoresces red.

N/A

3520-43-2

652.23

JC-1

5,5’,6,6’-tetrachloro-1,1’,3,3’-tetraethyl-benzimidazolylcarbocyanine Iodide

C₂₅H₂₇Cl₄IN₄

Brown red solid

N/A

No

Yes

>98% by HPLC

DMSO and DMF

-20°C

gel pack

12 months

Protect from light and moisture

cell

For cells, cell lines and tissues in culture till half confluency.