JC-1 for cell activation

Catalog number
1130-5
Name
JC-1 for cell activation
Size
5 mg
Price
338.00 EUR
Supplier

Details

Description
JC-1 is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. JC-1 monomer is in equilibrium with so called J-aggregates, which are favored at higher mitochondrial membrane potential. The monomer JC-1 has green fluorescence (lem = 527 nm), while the J-aggregates have red fluorescence (lem = 590 nm). Therefore, it has been possible to use fluorescence ratioing technique to study mitochondrial membrane potential. JC-1 is particularly useful for apoptosis studies. In apoptotic cells, the dye stays in the cytoplasm and fluoresces green, while in healthy cells, the dye aggregates in the mitochondria and fluoresces red.
Peptide sequence
N/A
CAS number
3520-43-2
Molecular weight
652.23
Category
JC-1
Other name
5,5’,6,6’-tetrachloro-1,1’,3,3’-tetraethyl-benzimidazolylcarbocyanine Iodide
Molecular formula
C₂₅H₂₇Cl₄IN₄
Physical appearance
Brown red solid
Supplied with
N/A
Is this a salt?
No
Is it cell-permeable?
Yes
Purification
>98% by HPLC
Reconstitute instructions
DMSO and DMF
Storage condition
-20°C
Shipping condition
gel pack
Maximum time for storage
12 months
Storage instructions
Protect from light and moisture
Tissue
cell
Additional description
For cells, cell lines and tissues in culture till half confluency.