PARP-1, human recombinant

Catalog number

4992-50

Size

50 μg

Price

495.00 EUR

Supplier

Biovision

Order

Details

Synonyms

Human PARP, PARP, h-PARP, rh-PARP, recombinant human PARP, PARPrecombinant PARP, PARP

Alternates names

Human PARP, PARP, h-PARP, rh-PARP, recombinant human PARP, PARPrecombinant PARP, PARP

Taglines

Involved in DNA repair and programmed cell death

Biomolecule/Target

NCBI Gene ID #.

142

NCBI Gene Symbol

PARP-1

Gene Source

Human

Accession #

P09874

Recombinant

Yes

Source

Baculovirus

Purity

N/A

Endotoxin Level

N/A

Activity (Specifications/test method)

N/A

Biological activity

1000 U/vial, specific activity = 20000 U/mg PARP1. 1U=10 fmol ADP-ribose incorporated into 5 μg immobilized histone in 30 min at room temperature. Note: Activity measurements are approximate values.

Results

N/A

Molecular Weight

116.0 kDa

Storage Temp.

-70°C

Shipping

gel pack

Shelf Life

1 year

Concentration

N/A

Appearance

Lyophilized protein

Physical form description

Lyophilized solid

Reconstitution Instructions

Spin tube in a microfuge for 15 sec to sediment lyophilized material. Carefully open the vial and add 100 μL dH₂O. Vortex gently for 20 sec (avoid air bubbles). Let stand for 5 min. Carefully triturate the sample 10-times using a pipetman (avoid air bubbles). Spin briefly in microfuge to consolidate. Upon reconstitution with 100 μL dH20, the final concentrations are as follows: 0.5 mg/mL PARP1 enzyme, 20 mM Tris, pH 8, 0.3 M NaCl, 0.1 mM EDTA, 1 mM DTT, plus lyophilization stabilizers.

Handling

Centrifuge the vial prior to opening.

Usage

For Research Use Only! Not to be used in humans

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Additional source

Recombinants or rec. proteins

Group

recombinants