Human CellExp™ Renin, Human Recombinant

Catalog number

6300-100

Size

100 μg

Price

746.00 EUR

Supplier

Biovision

Order

Details

Background

Renin is an aspartyl protease that is specific for only one substrate, angiotensinogen. Prorenin is a glycosylated aspartic protease that consists of 2 homologous lobes and is the precursor of renin. Prorenin exhibits a low level of enzymatic activity relative to renin. BioVision’s human renin is produced from the proenzyme prorenin by proteolytic cleavage of a 43 amino acid N-terminal prosegment using limited enzymatic digestion by immobilized trypsin and purified by affinity chromatography. Conversion to active renin is >99.99 percent.

Concentration

0.74 mg/ml

Molecular weight

40 kDa (contains 8x HIS tag at the CT)

Synonym

Human Recombinant HIS-tagged Renin

Other name

Angiotensinogenase, REN.

NCBI gene number

5972

NCBI gene

REN

Gene source

Human

NCBI number

P00797

Recombinant

Yes

Source

Human Embryonic Kidney (HEK) cells

Purification

≥95%

Tested applications

SDS-PAGE, N/A

Level of endotoxin

N/A

Tested activity

99.996 % active (< 17 ng/ml prorenin by ELISA)

Biological activity

N/A

Reesults

N/A

Binding ability

N/A

Unit

N/A

Storage condition

-80°C

Shipping under

gel pack

Physical appearance

Liquid

Physical properties

0.74 mg/ml solution in 0.05M Tris-HCl and 0.05 M NaCl, pH 8.0

How to reconstitute

N/A

Aa sequence

N/A

Before use

Centrifuge the vial prior to opening.

Notes

For Research Use Only! Not to be used in humans

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Additional source

Recombinants or rec. proteins

Group

recombinants