TMB, High Kinetics for cell activation

Catalog number
1216-100
Name
TMB, High Kinetics for cell activation
Size
100 ml
Price
181.00 EUR
Supplier

Details

Description
3,3',5,5'-Tetramethylbenzidine (TMB) has been shown to be a safe, sensitive substrate for the assay of horseradish peroxidase (HRP). Initially, in the presence of HRP and hydrogen peroxide, a one-electron oxidation product is formed. This compound, a cation free radical, is blue in color with an adsorption maximum at 653 nm. Further reaction with HRP/H₂O₂ or acidification of the radical with acid yields the diimine terminal oxidation product adsorbing light at 450 nm. The extinction coefficient of the radical (E653 nm = 3.9 x 10⁴ mol⁻¹ cm⁻¹) and diimine (E450 nm = 5.9 x 10⁴ mol⁻¹ cm⁻¹ ) provide a remarkably sensitive system for the assay of HRP and HRP labeled probes. TMBHK, available from BioVision Inc., is a highly sensitive single component reagent that is ready to use for the quantitative detection of HRP bound to a solid phase or in free solution. TMBHK is stable at room temperature for six months and is not sensitive to normal laboratory light. It is optimized with respect to TMB and hydrogen peroxide concentrations and yields a linear response with the concentrations of HRP commonly employed in immunologic assays.
Peptide sequence
N/A
CAS number
54827-17-7
Molecular weight
240.35
Category
Biochemicals
Other name
3,3',5,5'-Tetramethylbenzidine; TMBHK
Molecular formula
C₁₆H₂₀N₂
Physical appearance
Liquid
Supplied with
2.5 mMol L⁻¹ TMB and Hydrogen Peroxide in a proprietary buffer at pH 3.6.
Is this a salt?
No
Is it cell-permeable?
No
Purification
N/A
Reconstitute instructions
N/A
Storage condition
+4°C
Shipping condition
RT
Maximum time for storage
12 months
Storage instructions
Protect from light
Tissue
cell
Additional description
For cells, cell lines and tissues in culture till half confluency.