Human Recombinant TNF-beta/LTA

Catalog number

6376-50

Size

50 μg

Price

475.00 EUR

Supplier

Biovision

Order

Details

Synonyms

Human Recombinant TNF-beta/LTA

Alternates names

Lymphotoxin alpha, LT, TNFB, TNFSF1.

Taglines

Mediates a large variety of inflammatory, immunostimulatory, and antiviral responses

Biomolecule/Target

NCBI Gene ID #.

4049

NCBI Gene Symbol

LTA

Gene Source

Human

Accession #

P01374

Recombinant

Yes

Source

E. Coli

Purity

N/A

Endotoxin Level

N/A

Activity (Specifications/test method)

N/A

Biological activity

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is

Molecular Weight

20.9 kDa (192 aa, 35-205 aa + His-Tag)

Storage Temp.

-20°C

Shipping

gel pack

Shelf Life

1 year

Concentration

0.5 mg/ml

Appearance

Liquid

Physical form description

0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol.

Reconstitution Instructions

N/A

Handling

Centrifuge the vial prior to opening.

Usage

For Research Use Only! Not to be used in humans

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Additional source

Recombinants or rec. proteins

Group

recombinants

Gene

Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.