Human Recombinant DHFR

Catalog number

6382-100

Size

100 μg

Price

385.00 EUR

Supplier

Biovision

Order

Details

Synonyms

Human Recombinant DHFR

Alternates names

Dihydrofolate reductase.

Taglines

Reduces dihydrofolic acid to tetrahydrofolic acid

Biomolecule/Target

NCBI Gene ID #.

1719

NCBI Gene Symbol

DHFR

Gene Source

Human

Accession #

P00374

Recombinant

Yes

Source

E. Coli

Purity

N/A

Endotoxin Level

< 1.0 EU per 1 μg of protein (determined by LAL method)

Activity (Specifications/test method)

N/A

Biological activity

Specific activity is 1.5 - 2.5 units/ml and was obtained by measuring the oxidation of NADPH in absorbance at 340 nm during reaction.

Results

1.5 - 2.5 units/ml

Molecular Weight

23.6 kDa (207 aa, 1-187 aa + NT His-Tag)

Storage Temp.

-20°C

Shipping

gel pack

Shelf Life

1 year

Concentration

1 mg/ml

Appearance

Liquid

Physical form description

1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 2 mM DTT and 30% glycerol.

Reconstitution Instructions

N/A

Handling

Centrifuge the vial prior to opening.

Usage

For Research Use Only! Not to be used in humans

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Additional source

Recombinants or rec. proteins

Group

recombinants