Yeast recombinant Ubc13 (UBE2N)

Ubc13 is an E2 (ubiquitin conjugating enzyme) that is involved in the conjugation of ubiquitin to target substrates along with E1 and E3 enzymes. Ubc13 acts with the UEV protein Mms2 forming a heterodimeric complex that assembles K63-linked polyubiquitin chains utilized in nondegradative ubiquitin signaling pathways (DNA repair, signal transduction). The Ubc13/Mms2 complex is unique in that it has the ability to form free K63-linked polyubiquitin chains in solution in the absence of an E3. UBE2N mediates the transcription of some target genes and is believed to have a role in cell cycle progression; cellular differentiation and DNA repair mechanisms which ensure cell survival after DNA damage.

N/A

18.462 kDa with His Tag.

Yeast recombinant Ubc13 (UBE2N)

Ubiquitin-conjugating enzyme E2 N, Bendless-like ubiquitin-conjugating enzyme, Ubiquitin carrier protein N, Ubiquitin-protein ligase N, BLU, MGC8489, UbcH-ben, MGC131857.

851666

UBC13

Yeast

P52490

Yes

Saccharomyces cerevisiae

≥95%

SDS-PAGE, N/A

N/A

N/A

Typical enzyme concentration of 100 nM-5 mM is used for in vitro conjugation, depending on conditions.

N/A

N/A

N/A

-80°C

gel pack

Liquid

75 µL of a 40 µM solution (3 nmoles) in 50 mM HEPES, pH 7.5, 150 mM NaCl, 10% glycerol.

N/A

N/A

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Recombinants or rec. proteins

recombinants