Yeast recombinant Mms2 (UEV-2)

Catalog number

6434-3

Size

3 nmol

Price

365.00 EUR

Supplier

Biovision

Order

Details

Background

Mms2 is a UEV (ubiquitin E2 variant) protein that acts with the E2 enzyme Ubc13 in a heterodimeric complex which assembles K63-linked polyubiquitin chains utilized in non-degradative ubiquitin signaling pathways (DNA repair, signal transduction). The UEV proteins are structurally similar to E2 enzymes displaying the same fold, but lacking the active site cysteine of the E2. The Ubc13/Mms2 complex is unique in that it is has the ability to form free K63-linked polyubiquitin chains in solution in the absence of an E3.

Concentration

N/A

Molecular weight

15.544 kDa with His Tag.

Synonym

Yeast recombinant Mms2 (UEV-2)

Other name

UBE2V2

NCBI gene number

852793

NCBI gene

MMS2

Gene source

Yeast

NCBI number

P53152

Recombinant

Yes

Source

Saccharomyces cerevisiae

Purification

≥95%

Tested applications

RP-HPLC, N/A

Level of endotoxin

N/A

Tested activity

N/A

Biological activity

Protein concentration of 100 nM - 1 µM is recommended for in vitro conjugation.

Reesults

N/A

Binding ability

N/A

Unit

N/A

Storage condition

-80°C

Shipping under

gel pack

Physical appearance

Liquid

Physical properties

75 µL of a 40 µM solution (3 nmoles) in 50 mM Tris, pH 7.5, 150 mM NaCl, 10 mM DTT and 10% glycerol.

How to reconstitute

N/A

Aa sequence

N/A

Before use

Centrifuge the vial prior to opening.

Notes

For Research Use Only! Not to be used in humans

Additional source

Recombinants or rec. proteins

Group

recombinants