Human recombinant UBE2D2 (UbcH5b)

UBE2D2 (UbcH5b) is an E2 (ubiquitin conjugating enzyme) that is involved in the conjugation of ubiquitin to target substrates along with E1 and E3 enzymes. The UbcH5 family (including UbcH5a and UbcH5c, 88% and 89% sequence identity with UbcH5b, respectively) are the human homologues of the Saccharomyces cerevisiae UBC4/5 family (~79% sequence identity). Members of the UbcH5 family have been implicated in a number of inflammatory and cancer pathways, including the ubiquitylation of p53 (involving the E3 ligase Hdm2) and IκBα (involving the E3 ligase SCF).

N/A

17.729 kDa with His Tag.

Human recombinant UBE2D2 (UbcH5b)

Human recombinant UBE2D2 (UbcH5b), ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5), UBC4, Ubiquitin carrier protein D2, Ubiquitin-conjugating enzyme E2-17 kDa 2, Ubiquitin-protein ligase D2, UBCH5B, EC 6.3.2.19, E2(17)KB2, PUBC1, UBC4/5, UBC5B, UBCH4.

7322

UBE2D2

Human

P62837

Yes

N/A

≥95%

RP-HPLC, N/A

N/A

N/A

Protein concentration of 100 nM - 1 µM is recommended for in vitro conjugation.

N/A

N/A

N/A

-80°C

gel pack

Liquid

75 µL of a 40 µM solution (3 nmoles) in 50 mM Tris, pH 7.5, 150 mM NaCl, 10 mM DTT and 10% glycerol.

N/A

N/A

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Recombinants or rec. proteins

recombinants