Human recombinant UBE2E3 (UbcH9)

Ubiquitin-conjugating enzyme E2 E3 (UBE2E3 alias UbcH9) is a protein that in humans is encoded by the UBE2E3 gene. The deduced 207-amino acid protein shares over 94% amino acid identity with the UBC domains of class III E2s, UbcH6, UBE2E2, UbcM2, UbcM3, and UbcD2. But the N-terminal extension exhibited little homology among these, except for UbcM2, which showed 100% identity, and which is thought to be a mouse counterpart. The UBE2E3 form a thioester bond with ubiquitin (Ub) in an E1-dependent manner and mediates the transfer of ubiquitin from a ubiquitin-activating enzyme (E1) to a substrate protein or E3 ligase.

N/A

22.913 kDa

Human recombinant UBE2E3 (UbcH9)

Ubiquitin-conjugating enzyme E2E 3, Ubiquitin-conjugating enzyme E2-23 kDa, UBCH9, Ubiquitin-protein ligase E3, Ubiquitin-conjugating enzyme E2E3.

10477

UBE2E3

Human

Q969T4

Yes

N/A

≥95%

RP-HPLC, N/A

N/A

N/A

Protein concentration of 100 nM - 1 µM is recommended for in vitro conjugation.

N/A

N/A

N/A

-80°C

gel pack

Liquid

75 µL of a 40 µM solution (3 nmoles) in 20 mM Tris, pH 7.4, 150 mM NaCl, 10 mM DTT and 10% glycerol.

N/A

N/A

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Recombinants or rec. proteins

recombinants