Human CellExp™ Beta-NGF, Human Recombinant

NGF-beta has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. The presence of beta NGF in immune cells and endocrine cells as well as in the CNS limbic areas suggests that beta NGF may function as an intracellular messenger to regulate the body’s response to stress. This product is produced in a human cell expression system with serum-free, chemically defined media.

N/A

13 kDa, non-disulfide bonded homodimer, non-glycosylated.

Human Cellexp Human Recombinant Beta-NGF

Beta Polypeptide, NGF, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428

4803

NGF

Human

P01138

Yes

Human cell expressed

≥95%

SDS-PAGE, N/A

< 1.0 EU per 1 μg of protein

N/A

ED₅₀ is typically 1.5 to 8 ng/mL. The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

1.5 to 8 ng/ml

N/A

N/A

-80°C

gel pack

Lyophilized

Lyophilized from a PBS solution.

Reconstitute in sterile PBS containing 0.1% endotoxin-free, recombinant human serum albumin.

N/A

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Recombinants or rec. proteins

recombinants