Human CellExp™ GM-CSF, Human Recombinant

Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) is a growth factor that supports the in-vitro colony formation of granulocytes-macrophages progenitor cells. It is a pleiotropic cytokine and is produced as a response to immune or inflammatory stimuli by activated cells of the hematopoietic system such as T cells, B cells, macrophages, mast cells, fibroblasts and alveolar epithelial cells. It plays an important role in regulating the proliferation, differentiation, survival and activation of hematopoietic cells such as granulocytes and monocytes, neutrophiles, basophiles and eosonophoiles, erythroid cells, megakaryocytes and T cells. Human and mouse GM-CSF have about 56% homology and are species specific. Human GM-CSF is not active on mouse cells and vice versa. It is active on canine and feline cells.

N/A

15 to 36 kDa, monomer, glycosylated

Human Cellexp Human Recombinant GM-CSF

CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim, MGC131935, MGC138897.

1437

CSF2

Human

P04141

Yes

Human 293 cell expressed

≥95%

SDS-PAGE, N/A

< 1.0 EU per 1 μg of protein

N/A

ED₅₀ is typically 0.4 to 2 ng/mL. The specific activity was determined by the dose- Dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic Indicator cell line).

0.4 to 2 ng/ml

N/A

N/A

-80°C

gel pack

Lyophilized

Lyophilized from a PBS solution.

Reconstitute in sterile PBS containing 0.1% endotoxin-free, recombinant human serum albumin.

N/A

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim. Reconstitution It is recommended that sterile ddH2O containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of not less than 1 mg/mL of the cytokine.

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Recombinants or rec. proteins

recombinants