Human CellExp™ IL-1beta, Human Recombinant

Interleukin-1beta is produced by activated macrophages. IL-1beta stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL1beta proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. In comparison with the E. coli expressed protein, IL-1 beta is 50% more potent in promoting human CD4+ T cell differentiation into Th17 cells. This cytokine is produced in a serumfree, chemically defined media.

N/A

18 and 25 kDa, monomer, glycosylated

Human Cellexp Human Recombinant IL-1beta

Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL1F2, IL-1 beta.

3553

IL1B

Human

P01584

Yes

Human 293 cell expressed

≥95%

SDS-PAGE, N/A

< 1.0 EU per 1 μg of protein

N/A

ED₅₀ is typically 0.02 to 0.08 ng/mL. The specific activity was determined by the dose-dependent stimulation of the proliferation of mouse D10S cells.

0.02 to 0.08 ng/ml

N/A

N/A

-80°C

gel pack

Lyophilized

Lyophilized from a PBS solution.

Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

N/A

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Recombinants or rec. proteins

recombinants