Human CellExp™ IL-17A, Human Recombinant

Interleukin-17 is a proinflammatory cytokine produced by activated T cells. IL-17 regulates the activities of NF-kappa B and mitogen-activated protein kinases. IL17 can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The original described IL-17 protein, now known as IL-17A, was identified from a CD4+ T cells DNA library. IL-17 can be induced from primary peripheral blood CD4+ T cells upon stimulation. Supernatant from cells transfected with IL-17 induced IL-16 and IL-18 production and enhanced the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts.

N/A

30 to 35 kDa, homodimer, glycosylated

Human Cellexp Human Recombinant IL-17A

CTLA-8, IL-17, IL-17A, Cytotoxic T-lymphocyte-associated antigen 8.

3605

IL17A

Human

Q16552

Yes

Human 293 cell expressed

> 95%

SDS-PAGE, N/A

< 1.0 EU per 1 μg of protein

N/A

ED₅₀ is typically 5 to 25 ng/mL. The specific activity was determined by the dose-dependent induction of IL-6 secretion from NDHF Adult fibroblasts.

5 to 25 ng/ml

N/A

N/A

-80°C

gel pack

Lyophilized

Lyophilized in 10 mM Tris, pH 7.4 and 250 mM NaCl.

Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

N/A

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Recombinants or rec. proteins

recombinants