Human CellExp™ PDGF-AA, Human Recombinant

PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGF is stored in platelet alpha-granules and released upon platelet activation. The PDGF is involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGF have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB.

N/A

35, 40 and 45 kDa, homodimer, glycosylated

Human Cellexp Human Recombinant PDGF-AA

Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-AA, PDGF-1.

5154

PDGFA

Human

P04085

Yes

Human 293 cell expressed

> 95%

SDS-PAGE, N/A

< 1.0 EU per 1 μg of protein

N/A

ED₅₀ is typically 200 to 1000 ng/mL. The specific activity was determined by the dose-dependent stimulation of the proliferation of 3T3 cells.

200 to 1000 ng/ml

N/A

N/A

-80°C

gel pack

Lyophilized

Lyophilized from a solution of 10 mM AcOH.

Reconstitute in sterile PBS containing 10 mM AcOH.

N/A

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Recombinants or rec. proteins

recombinants