Human CellExp™ VEGF 165, Human Recombinant

VEGF is an important signaling protein involved in both vasculogenesis and angiogenesis. As its name implies, VEGF activity has been mostly studied on cells of the vascular endothelium, although it does have effects on a number of other cell types (e.g. stimulation monocyte/ macrophage migration, neurons, cancer cells, kidney epithelial cells).VEGF mediates increased vascular permeability, induces angiogenesis, vasculogenesis and endothelial cell growth, promotes cell migration, and inhibits apoptosis. In vitro, VEGF has been shown to stimulate endothelial cell mitogenesis and cell migration. VEGF is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor. Elevated levels of this protein are linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy.

N/A

45 kDa, homodimer, glycosylated

Human Cellexp Human Recombinant VEGF 165

Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen, VEGF, MGC70609.

7422

VEGFA

Human

P15692

Yes

Human 293 cell expressed

> 95%

SDS-PAGE, N/A

< 1.0 EU per 1 μg of protein

N/A

ED₅₀ is typically 2 to 10 ng/mL. The specific activity was determined by the dose-dependent stimulation of the proliferation of HUVEC cells (Human Umbilical Vein Endothelial Cells).

2 to 10 ng/ml

N/A

N/A

-80°C

gel pack

Lyophilized

Lyophilized in PBS.

Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

N/A

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Recombinants or rec. proteins

recombinants