Protein A/G-FITC

Catalog number
6504-1
Name
Protein A/G-FITC
Size
1 mg
Price
595.00 EUR
Supplier

Details

Synonyms
Protein A/G
Alternates names
Protein A/G
Taglines
Genetically engineered protein ideal for purification of polyclonal or monoclonal IgG antibodies
Biomolecule/Target
NA
NCBI Gene ID #.
N/A
NCBI Gene Symbol
N/A
Gene Source
N/A
Accession #
N/A
Recombinant
Yes
Source
E. coli
Purity
≥97%
Activity (Specifications/test method)
N/A
Biological activity
N/A
Results
N/A
Molecular Weight
59.7 kDa
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized
Physical form description
Lyophilized with no additives
Reconstitution Instructions
Reconstitution in H₂O to a concentration of 5 mg/ml gives a clear solution.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.
Conjugation
Anti-FITC Antibody