GSK-3β, human recombinant
Catalog number
7004-100
Name
GSK-3β, human recombinant
Size
100 μg
Price
595.00 EUR
Supplier
Details
Synonyms
GSK, Glycogen synthase kinase-3 (GSK-3)
Alternates names
GSK, Glycogen synthase kinase-3 (GSK-3)
Taglines
A phosphorylating and an inactivating agent of glycogen synthase
Biomolecule/Target
NA
NCBI Gene ID #.
2932
NCBI Gene Symbol
GSK3B
Gene Source
Human
Accession #
P49841
Recombinant
Yes
Source
E. coli.
Purity
N/A
Endotoxin Level
N/A
Activity (Specifications/test method)
Activity of GSK-3β was determined by an in vitro kinase assay using 100 ng Tau as substrate and 1 µg of the recombinant GSK-3β in a 20 µl reaction containing 25 mM Tris-HCl, pH 7.5, 5 mM β-glycerol phosphate, 12 mM MgCl₂, 2 mM DTT, 0.1 M Na3VO4, and 200 µM ATP. After 30 minutes incubation at 30°C, phosphorylation of Tau by the recombinant GSK-3β was determined by Western blot using a phospho-Tau antibody.
Biological activity
NA
Results
N/A
Molecular Weight
53.0-56.0 kDa
Storage Temp.
-70°C
Shipping
dry ice
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized powder
Physical form description
Lyophilized from 2 mg/ml in 0.1 M NH4CO3
Reconstitution Instructions
Reconstitute to 1 mg/ml in PBS containing 15% glycerol. The solution should be stored at –70°C, stable for up to 3 months.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants
