Human CellExp™ IL-4 Rα, Human Recombinant
Catalog number
7101-50
Name
Human CellExp™ IL-4 Rα, Human Recombinant
Size
50 μg
Price
882.00 EUR
Supplier
Details
Target
IL-4 Rα
Background
IL-4 can signal through type I and type II receptor complexes, which share a common gamma chain (γc). The type I receptor contains in addition to the γc an IL-4Rα subunit, whereas the type II receptor contains the IL-13Rα. The secreted extracellular domain of IL-4Rα, called sIL-4Rα, binds IL-4 and antagonizes its activity. It plays an important role in regulating the differentiation of naïve CD4+ T cells and class switching to IgG1 and IgE. Recombinant human sIL-4Rα is a 209 amino acid protein which corresponds to the entire extracellular domain of IL- 4Rα.
Concentration
N/A
Molecular weight
~24 kDa
Synonym
soluble IL-4 receptor alpha, CD124
Other name
soluble IL-4 receptor alpha, CD124
NCBI gene number
3566
NCBI gene
IL4RA
Gene source
Human
NCBI number
P24394
Recombinant
Yes
Source
HEK293 cells
Purification
≥98%
Tested applications
SDS-PAGE, HPLC
Level of endotoxin
< 0.1 ng/μg of protein (
Tested activity
Determined by its ability to inhibit the IL-4-dependent proliferation of human TF-1 cells. The expected ED50 is ≤ 5.0 ng/ml (in the presence of 0.5 ng/ml of hIL-4), corresponding to a specific activity of ≥ 2 x 105 units/mg.
Biological activity
Determined by its ability to inhibit the IL-4-dependent proliferation of human TF-1 cells. The expected ED50 is ≤ 5.0 ng/ml (in the presence of 0.5 ng/ml of hIL-4), corresponding to a specific activity of ≥ 2 x 105 units/mg.
Reesults
N/A
Binding ability
N/A
Unit
N/A
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized powder
Physical properties
Sterile filtered through a 0.2 micron filter. Lyophilized from 1x PBS pH 7.0.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to - 80°C
Aa sequence
GNMKVLQEPT CVSDYMSIST CEWKMNGPTN CSTELRLLYQ LVFLLSEAHT CIPENNGGAG CVCHLLMDDV VSADNYTLDL WAGQQLLWKG SFKPSEHVKP RAPGNLTVHT NVSDTLLLTW SNPYPPDNYL YNHLTYAVNI WSENDPADFR IYNVTYLEPS LRIAASTLKS GISYRARVRA WAQCYNTTWS EWSPSTKWHN SYREPFEQH
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants
