Calcein AM for cell activation

A cell-permeable, non-fluorescent acetoxymethyl ester derivative of Calcein that gets hydrolyzed inside the cell by esterases. The loss of the acetoxymethyl group also enables calcein to readily bind intracellular calcium resulting in a strong yellowish-green fluorescence. It is the best probe for cell viablilty and cell adhesion due to its greater retention in cells as well and its pH insensitivity in physiological range.

N/A

148504-34-1

994.86

Biochemicals

Glycine,N,N'-[[3',6'-bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl]bis(methylene)]bis[N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-,bis[(acetyloxy)methyl] ester; Calcein acetoxymethyl ester

C₄₆H₄₆N₂O₂₃

Off-white solid

N/A

No

Yes

≥95% by HPLC

DMSO (5 mg/ml)

-20°C

gel pack

24 months

Protect from air and light

cell

For cells, cell lines and tissues in culture till half confluency.