sFas Ligand, human recombinant
Catalog number
7140-10
Name
sFas Ligand, human recombinant
Size
10 μg
Price
335.00 EUR
Supplier
Details
Synonyms
soluble Fas Ligand (sFasL), TNFSF6, CD95L, Apo I Ligand, APTL
Alternates names
soluble Fas Ligand (sFasL), TNFSF6, CD95L, Apo I Ligand, APTL
Taglines
Leads to down-regulation of the immune response
Biomolecule/Target
sFas Ligand
NCBI Gene ID #.
355
NCBI Gene Symbol
FASL
Gene Source
Human
Accession #
P25445
Recombinant
Yes
Source
CHO cells
Purity
N/A
Endotoxin Level
< 0.1 ng/μg of protein (
Activity (Specifications/test method)
Determined by its ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The ED50 for this effect is ≤10.0 ng/ml, corresponding to a specific activity of ≥ 1 x 105 units/mg.
Biological activity
Determined by its ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The ED50 for this effect is ≤10.0 ng/ml, corresponding to a specific activity of ≥ 1 x 105 units/mg.
Results
N/A
Molecular Weight
17.9 kDa
Storage Temp.
-20°C
Shipping
Gel Pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized powder
Physical form description
Sterile filtered through a 0.2 micron filter. Lyophilized from 0.5x PBS, pH 7.5.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Description
FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.
Additional source
Recombinants or rec. proteins
Group
recombinants
