Puromycin Dihydrochloride for cell activation

A nucleoside antibiotic. It inhibits protein synthesis by disrupting peptide transfer on ribosomes causing premature chain termination during translation. It is a potent translational inhibitor in both prokaryotic and eukaryotic cells. Resistance to puromycin is conferred by the puromycin N-acetyl-transferase gene (pac) from Streptomyces. Puromycin has a fast mode of action, causing rapid cell death at low antibiotic concentrations. Adherent mammalian cells are sensitive to concentrations of 2 to 5 µg/ml, while cells in suspension are sensitive to concentrations as low as 0.5 to 2 µg/ml.

N/A

58-58-2

544.43

Biochemicals

3′-[α-Amino-p-methoxyhydrocinnamamido]-3′-deoxy-N,N-dimethyladenosine dihydrochloride

C₂₂H₂₉N₇O₅ · 2HCl

White to off-white solid

N/A

No

No

≥98% by HPLC

H₂O (~50 mg/ml)

-20°C

gel pack

24 months

Protect from air and moisture

cell

For cells, cell lines and tissues in culture till half confluency.