Human CellExp™ SHH, human recombinant

SHH

Sonic hedgehog protein (SHH) also known as HHG-1, which belongs to the hedgehog family. SHH is expressed in fetal intestine, liver, lung, and kidney and not expressed in adult tissues. Sonic hedgehog protein can be cleavage into sonic hedgehog protein N-product and sonic hedgehog protein C-product by autolysis. SHH binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development. The C-terminal domain displays an auto-proteolysis activity and a cholesterol transferase activity. The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.

N/A

This protein is fused with 6 × His tag at the C-terminus, has a calculated MW of 20.4 kDa. The predicted N-terminus is Cys 24. DTT-reduced protein migrates as 22 kDa.

SHH, HHG-1, Sonic hedgehog, HLP3, HPE3, SMMCI, TPT, TPTPS

SHH, HHG-1, Sonic hedgehog, HLP3, HPE3, SMMCI, TPT, TPTPS

6469

SHH

Human

Q15465

Yes

HEK 293 cells

≥95%

SDS-PAGE, HPLC

< 0.1 ng/μg of protein (

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 1.5-10 μg/mL.

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 1.5-10 μg/mL.

N/A

N/A

N/A

-20°C

Gel Pack

Lyophilized powder

Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

N/A

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Recombinants or rec. proteins

recombinants