Human CellExp™ CCL6, mouse recombinant

CCL6

Chemokine (C-C motif) ligand 6 (CCL6), a member of the CC chemokine family, was initially identified as a transcript that is induced in bone marrow cells upon stimulation with GM-CSF. CCL6 has only been identified in rodents. A human homologue for mouse CCL6 has not been identified. The mCCL6 cDNA encodes a 116 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to yield a 95 amino acid residue mature protein. In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, where it is strongly induced upon GM-CSF stimulation. Mouse CCL6 expression was also shown to be strongly induced by IL-4 in mouse macrophages and by cytokine IL-13 in mouse lung. Mouse CCL6 is located on chromosome 11. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.

N/A

This protein is fused with a C-terminal 6 × His tag, has a calculated MW of 11.4 kDa expressed. The predicted N-terminus is Gly22. Protein migrates as 14 kDa in reduced SDS-PAGE.

CCL6, SCYA6, C10, MRP-1

CCL6, SCYA6, C10, MRP-1

20305

CCL6

Mouse

P27784

Yes

HEK 293 cells

≥95%

SDS-PAGE, HPLC

< 0.1 ng/μg of protein (

Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR1.The ED50 for this effect is typically 0.01-0.15 μg/mL.

Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR1.The ED50 for this effect is typically 0.01-0.15 μg/mL.

N/A

N/A

N/A

-20°C

Gel Pack

Lyophilized powder

Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

N/A

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Mus musculus

Recombinants or rec. proteins

recombinants