Human CellExp™ VEGF-C, human recombinant

VEGF-C

Vascular endothelial growth factor C is also known as VEGFC, Flt4-L and VRP; it contains the C-terminal propeptide which has an unusual structure with tandemly repeated cysteine-rich motifs. Upon biosynthesis, VEGFC is secreted as a non-covalent momodimer in an anti-parallel fashion. VEGFC is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family, is active in angiogenesis, lymphangiogenesis and endothelial cell growth and survival, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms that bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. The structure and function of this protein is similar to those of vascular endothelial growth factor D (VEGF-D). VEGFC may function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Overexpression of VEGF-C causes lymphatics to enlarge possibly facilitates metastasis.

N/A

The protein is fused with 6×His tag at the C-terminus, has a calculated MW of 15 kDa. The predicted N-terminus is Thr 103. DTT-reduced Protein migrates as 18-23 kDa due to glycosylation.

VEGFC, Flt4-L, VRP

VEGFC, Flt4-L, VRP

7424

VEGF-C

Human

P49767

Yes

HEK 293 cells

≥97%

SDS-PAGE, HPLC

< 0.1 ng/μg of protein (

Measured in a cell proliferation assay using HMVEC human microvascular endothelial cells. The ED50 for this effect is typically 0.15-0.7 μg/ml.

Measured in a cell proliferation assay using HMVEC human microvascular endothelial cells. The ED50 for this effect is typically 0.15-0.7 μg/ml.

N/A

N/A

N/A

-20°C

Gel Pack

Lyophilized powder

Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

N/A

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Recombinants or rec. proteins

recombinants