Human CellExp™ PCSK9, murine recombinant

PCSK9

Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).

N/A

This protein is fused with 6×His tag at the N-terminus, has a calculated MW of 72 kDa. The predicted N-terminus is Gln 35. DTT-reduced Protein migrates as 20 kDa and 64 kDa due to glycosylation.

PCSK9, FH3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, Proprotein convertase subtilisin/kexin type 9

PCSK9, FH3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, Proprotein convertase subtilisin/kexin type 9

100102

PCSK9

Mouse

Q80W65

Yes

HEK 293 cells

≥97%

SDS-PAGE, N/A

< 0.1 ng/μg of protein (

Measured by its binding ability in a functional ELISA Immobilized mouse PCSK9 at 10 μg/ml (100 μl/well) can bind biotinylated rhLDLR. The EC50 of biotinylated human LDLR is 0.1 μg/ml.

Measured by its binding ability in a functional ELISA Immobilized mouse PCSK9 at 10 μg/ml (100 μl/well) can bind biotinylated rhLDLR. The EC50 of biotinylated human LDLR is 0.1 μg/ml.

N/A

N/A

N/A

-20°C

Gel Pack

Lyophilized powder

Sterile filtered through a 0.22 micron filter. Lyophilized from 1 x PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 100 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

N/A

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Recombinants or rec. proteins

recombinants