Human CellExp™ LIF, human recombinant

LIF

Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.

N/A

Calculated MW of 19.9 kDa with Gly-Pro at the N- terminus. The predicted N-terminus is Ser 23. DTT-reduced Protein migrates as 35-45 kDa.

LIF, CDF, DIA, HILDA, MLPLI

LIF, CDF, DIA, HILDA, MLPLI

3976

LIF

Human

P15018

Yes

HEK 293 cells

≥95%

SDS-PAGE, N/A

< 0.1 ng/μg of protein (

Determined by its ability to stimulate the proliferation of human TF-1 cells. The ED50 1X10^7 units/mg.

Determined by its ability to stimulate the proliferation of human TF-1 cells. The ED50 1X10^7 units/mg.

N/A

N/A

N/A

-20°C

Gel Pack

Lyophilized powder

Sterile filtered through a 0.22 micron filter. Lyophilized from 1 x PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

N/A

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Recombinants or rec. proteins

recombinants