Human CellExp™ Angiopoietin-2, human recombinant

Angiopoietin-2

Angiopoietin-2, also known as ANGPT2, AGPT2, ANG2, and is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Ang2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel Palade bodies. Both Ang2 and the related Angiopoietin1 (Ang1) are ligands for the receptor tyrosine kinase Tie 2. Ang2 functions as a proangiogenic factor, although it can also induce EC death and vessel regression. Upon its release from quiescent EC, it regulates vascular remodeling by promoting EC survival, proliferation, and migration and destabilizing the interaction between EC and perivascular cells. Ang2 is required for postnatal vascular remodeling, and it cooperates with Ang1 during lymphatic vessel development. It mediates the upregulation of ICAM1 and VCAM1 on EC, which facilitates the adhesion of leukocytes during inflammation. Ang2 competitively inhibit Ang1-induced endothelial cell responses mediated by Tie2, and reduces vascular integrity. But the role of Ang2 is controversial since the opposite outcomes have been reported in other studies. Over-expression of Ang2 disrupts the vascular remodeling, induces endothelial cell apoptosis, and may play an important regulating role in tumor angiogenesis. Ang2 also promotes the neuronal differentiation and migration of sub ventricular zone progenitor cells.

N/A

This protein is fused with a 6×his tag at C-terminus and the protein has a calculated MW of 50.1 kDa. The predicted N-terminus is Asp68 & Tyr19. DTT-reduced protein migrates as 66-90 kDa in SDS-PAGE due to glycosylation.

ANGPT2, AGPT2, ANG2, Angiopoietin-2

ANGPT2, AGPT2, ANG2, Angiopoietin-2

285

ANGPT1

Human

O15123

Yes

HEK293 cells

≥90%

SDS-PAGE, N/A

N/A

Measured by its ability to activate Tie2 in human Tie2 transfected C6 rat glial cells. 0.2 μg/mL of rhANGPT2 significantly induces phosphorylation of human Tie2. Measured in a competitive binding assay. When Tie2 Fc Chimera is immobilized, rhAng2 inhibits binding of biotinylated rhAng-2.

Measured by its ability to activate Tie2 in human Tie2 transfected C6 rat glial cells. 0.2 μg/mL of rhANGPT2 significantly induces phosphorylation of human Tie2. Measured in a competitive binding assay. When Tie2 Fc Chimera is immobilized, rhAng2 inhibits binding of biotinylated rhAng-2.

N/A

N/A

-20°C

Gel Pack

Lyophilized

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

N/A

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Recombinants or rec. proteins

recombinants