Human CellExp™ SERPIND1, human recombinant

SERPIN D1

Serpin D1 is also known as Heparin cofactor 2 (HCF2), Protease inhibitor leuserpin-2 (HLS2), Heparin cofactor II (HC-II), which belongs to the serpin family. HCF2 is expressed predominantly in liver. Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, SerpinD1 / HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). In a glycosaminoglycan-independent manner, serpinD1 / HC-II also inhibits chymotrypsin. Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils.

N/A

This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 55.7 kDa. The predicted N-terminus is Gly 20. DTT-reduced Protein migrates as 65-70 kDa due to glycosylation.

SerpinD1, HCF2, HLS2, HC-II

SerpinD1, HCF2, HLS2, HC-II

3053

SERPIND1

Human

P05546

Yes

HEK293 cells

≥95%

SDS-PAGE, N/A

N/A

Measured by its ability to inhibit Recombinant Human Coagulation Factor II/Thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC. The IC50 value is

Measured by its ability to inhibit Recombinant Human Coagulation Factor II/Thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC. The IC50 value is

N/A

N/A

-20°C

Gel Pack

Lyophilized

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

N/A

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Recombinants or rec. proteins

recombinants