Human CellExp™ Kallikrein-3, human recombinant

Catalog number

7415-10

Size

10 μg

Price

289.00 EUR

Supplier

Biovision

Order

Details

Target

Kallikrein-3

Background

Kallikrein-3 (KLK3) is also known as Prostate-specific antigen (PSA), APS, Gamma-seminoprotein (Seminin), P-30 antigen, Semenogelase, is a glycoprotein enzyme, is a member of the kallikrein-related peptidase family and is secreted by the epithelial cells of the prostate gland. The preferential cleavage site of KLK3 is “-Tyr-|-Xaa-“, the catalytic activity is inhibited by SERPINA5 and is strongly inhibited by Zn2+. KLK3 hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

Concentration

N/A

Molecular weight

This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 27.7 kDa. The predicted N-terminus is Ala 18. DTT-reduced Protein migrates as 36 kDa due to glycosylation.

Synonym

PSA, KLK3, KLKB1, Kallikrein-3, APS, KLK2A1, PSA, Hk3, Seminin, Semenogelase

Other name

PSA, KLK3, KLKB1, Kallikrein-3, APS, KLK2A1, PSA, Hk3, Seminin, Semenogelase

NCBI gene number

3818

NCBI gene

KLK3

Gene source

Human

NCBI number

P03952

Recombinant

Yes

Source

HEK293 cells

Purification

≥92%

Tested applications

SDS-PAGE, N/A

Tested activity

N/A

Biological activity

Measured by its ability to cleave the colorimetric peptide substrate, Succinyl – Arg – Pro – Tyr – p – Nitroanilide (Suc-RPY-pNA). The specific activity is >60 pmoles / min / μg.

Reesults

Measured by its ability to cleave the colorimetric peptide substrate, Succinyl – Arg – Pro – Tyr – p – Nitroanilide (Suc-RPY-pNA). The specific activity is >60 pmoles / min / μg.

Binding ability

N/A

Unit

N/A

Storage condition

-20°C

Shipping under

Gel Pack

Physical appearance

Lyophilized

Physical properties

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.

How to reconstitute

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Aa sequence

N/A

Before use

Centrifuge the vial prior to opening.

Notes

For Research Use Only! Not to be used in humans

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Additional source

Recombinants or rec. proteins

Group

recombinants