Human CellExp™ Kallikrein-4, human recombinant

Catalog number

7416-10

Size

10 μg

Price

278.00 EUR

Supplier

Biovision

Order

Details

Target

Kallikrein-4

Background

Kallikrein-4 (KLK4) is also known as Enamel matrix serine proteinase 1 (EMSP1), Kallikrein-like protein 1 (KLK-L1), Prostase, Serine protease 17 (PRSS17), PSTS, which belongs to the peptidase S1 family and Kallikrein subfamily. KLK4 contains one peptidase S1 domain. KLK4 is expressed in prostate and involved in enamel formation. Defects in Kallikrein-4 / KLK4 are the cause of amelogenesis imperfecta hypomaturation type 2A1 (AI2A1) which is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions.

Concentration

N/A

Molecular weight

This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 25.2 kDa. The predicted N-terminus is Ser 27. DTT-reduced Protein migrates as 30-32 kDa due to glycosylation.

Synonym

KLK4, Kallikrein-4, Prostate, KLK-L1, EMSP1, PRSS17, PSTS

Other name

KLK4, Kallikrein-4, Prostate, KLK-L1, EMSP1, PRSS17, PSTS

NCBI gene number

9622

NCBI gene

KLK4

Gene source

Human

NCBI number

Q9Y5K2

Recombinant

Yes

Source

HEK293 cells

Purification

≥92%

Tested applications

SDS-PAGE, N/A

Tested activity

N/A

Biological activity

Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >200 pmoles / min / μg.

Reesults

Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >200 pmoles / min / μg.

Binding ability

N/A

Unit

N/A

Storage condition

-20°C

Shipping under

Gel Pack

Physical appearance

Lyophilized

Physical properties

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

How to reconstitute

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Aa sequence

N/A

Before use

Centrifuge the vial prior to opening.

Notes

For Research Use Only! Not to be used in humans

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Additional source

Recombinants or rec. proteins

Group

recombinants