Human CellExp™ Kallikrein-11, human recombinant

Kallikrein-11

Kallikrein 11 (KLK11) is also known as Hippostasin, Serine protease 20, Trypsin-like protease, PRSS20, TLSP, which belongs to the peptidase S1 family and Kallikrein subfamily. KLK11 contains one peptidase S1 domain. KLK11 is expressed in brain, skin and prostate. Isoform 1 is expressed preferentially in brain. Isoform 2 is expressed in prostate. Present in seminal plasma at concentrations ranging from 2 to 37 µg/mL (at protein level). Kallikrein 11 /KLK11 is a possible multifunctional protease and efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. KLK11 cleaves synthetic peptides after arginine but not lysine residues.

N/A

This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 26.5 kDa. The predicted N-terminus is Glu 19. DTT-reduced Protein migrates as 35-45 kDa due to glycosylation.

KLK11, Kallikrein-11, Hippostasin, hK11, PRSS20, TLSP

KLK11, Kallikrein-11, Hippostasin, hK11, PRSS20, TLSP

11012

KLK11

Human

Q9UBX7

Yes

HEK293 cells

≥95%

SDS-PAGE, N/A

N/A

Measured by its ability to cleave a colorimetric peptide substrate D-Val-Leu-Lys-ThioBenzyl ester (VLK-SBzl), in the presence of 5,5’ Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999). J. Biol. Chem. 274, 30468. The specific activity is > 300 pmoles / min / µg.

Measured by its ability to cleave a colorimetric peptide substrate D-Val-Leu-Lys-ThioBenzyl ester (VLK-SBzl), in the presence of 5,5’ Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999). J. Biol. Chem. 274, 30468. The specific activity is > 300 pmoles / min / µg.

N/A

N/A

-20°C

Gel Pack

Lyophilized

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 2 mM CaCl2, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

N/A

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Recombinants or rec. proteins

recombinants