Human CellExp™ SERPIN A3, human recombinant

Catalog number

7419-10

Size

10 μg

Price

289.00 EUR

Supplier

Biovision

Order

Details

Target

SERPIN A3

Background

Serpin A3 is also known as Alpha-1-antichymotrypsin (ACT or AACT), Cell growth-inhibiting gene 24/25 protein, which belongs to the serpin family. Serpin A3 can bind DNA. Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.

Concentration

N/A

Molecular weight

This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 46 kDa. The predicted N-terminus is His 23. DTT-reduced Protein migrates as 55-70 kDa due to glycosylation.

Synonym

SERPINA3, ACT, AACT, GIG24, GIG25

Other name

SERPINA3, ACT, AACT, GIG24, GIG25

NCBI gene number

NCBI gene

SERPINA3

Gene source

Human

NCBI number

P01011

Recombinant

Yes

Source

HEK293 cells

Purification

≥95%

Tested applications

SDS-PAGE, N/A

Tested activity

N/A

Biological activity

Measured by its ability to inhibit chymotrypsin cleavage of a fluorogenic peptide substrate MCA-RPKPVE-Nval-WRK (Dnp)-NH2. The IC50 value is

Reesults

Measured by its ability to inhibit chymotrypsin cleavage of a fluorogenic peptide substrate MCA-RPKPVE-Nval-WRK (Dnp)-NH2. The IC50 value is

Binding ability

N/A

Unit

N/A

Storage condition

-20°C

Shipping under

Gel Pack

Physical appearance

Lyophilized

Physical properties

Lyophilized from 0.22 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.

How to reconstitute

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Aa sequence

N/A

Before use

Centrifuge the vial prior to opening.

Notes

For Research Use Only! Not to be used in humans

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Additional source

Recombinants or rec. proteins

Group

recombinants