Human CellExp™ PD-L1 /CD274 /B7-H1, mouse recombinant

PD-L1 /CD274 /B7-H1

Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.

N/A

This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 25.6 kDa. The predicted N-terminus is Phe 19. DTT-reduced Protein migrates as 40-50 kDa due to glycosylation.

PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1

PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1

60533

CD274

Mouse

Q9EP73

Yes

HEK293 cells

≥95%

SDS-PAGE, N/A

N/A

Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse PDL1 at 1 μg/ml (100 μl/well) can bind biotinylated Mouse PDCD1 / PD1 with a linear range of 16 - 500 ng/ml.

Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse PDL1 at 1 μg/ml (100 μl/well) can bind biotinylated Mouse PDCD1 / PD1 with a linear range of 16 - 500 ng/ml.

N/A

N/A

-20°C

Gel Pack

Lyophilized

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

N/A

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Mus musculus

Recombinants or rec. proteins

recombinants