Human CellExp™ PD-L1 /CD274 /B7-H1, human recombinant

PD-L1 /CD274 /B7-H1

Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.

N/A

This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 26 kDa. The predicted N-terminus is Phe 19. DTT-reduced Protein migrates as 35-42 kDa due to glycosylation.

PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1

PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1

29126

CD274

Human

Q9NZQ7

Yes

HEK293 cells

≥95%

SDS-PAGE, N/A

N/A

Measured by its binding ability in a functional ELISA. Immobilized rh PD-L1 / B7-H1 (Catalog # 7429) at 2 μg/ml (100 μl/well) can bind rhPD1/PDCD1 Fc Chimera with a linear range of 1-10 μg/ml. Measured by its ability to inhibit anti­CD3 antibody induced IL­2 secretion in human T lymphocytes. The ED50 for this effect is typically 0.6-5 μg/ml.

Measured by its binding ability in a functional ELISA. Immobilized rh PD-L1 / B7-H1 (Catalog # 7429) at 2 μg/ml (100 μl/well) can bind rhPD1/PDCD1 Fc Chimera with a linear range of 1-10 μg/ml. Measured by its ability to inhibit anti­CD3 antibody induced IL­2 secretion in human T lymphocytes. The ED50 for this effect is typically 0.6-5 μg/ml.

N/A

N/A

-20°C

Gel Pack

Lyophilized

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

N/A

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Recombinants or rec. proteins

recombinants