Human CellExp™ SFRP1/SARP2, human recombinant

SFRP1/SARP2

Secreted frizzled-related protein 1 (SFRP1) also known as Secreted apoptosis-related protein 2 (SARP2), FRP, FRP1, is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The Secreted frizzled-related protein (SFRP) family consists of five secreted glycoproteins in humans (SFRP1, SFRP2, SFRP3, SFRP4, SFRP5) that act as extracellular signaling ligands. SFRP1 / SARP-2 is widely expressed and absent from lung, liver and peripheral blood leukocytes, and with the highest levels in heart and fetal kidney. SARP2 / SFRP-1 is a 35 kDa prototypical member of the SFRP family. SARP2 / FRP-1 acts as a biphasic modulator of Wnt signaling, counteracting Wnt-induced effects at high concentrations and promoting them at lower concentrations.

N/A

This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 33.4 kDa. The predicted N-terminus is Ser 32. DTT-reduced Protein migrates as 38 kDa due to glycosylation.

SFRP1, FRP, FRP1, SARP2, FrzA

SFRP1, FRP, FRP1, SARP2, FrzA

6422

SFRP1

Human

Q8N474

Yes

HEK293 cells

≥92%

SDS-PAGE, N/A

N/A

Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED50 for this effect is typically 2.5 - 10 µg/ml.

Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED50 for this effect is typically 2.5 - 10 µg/ml.

N/A

N/A

-20°C

Gel Pack

Lyophilized

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

N/A

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Recombinants or rec. proteins

recombinants