Human CellExp™ Carbonic Anhydrase 4/CA4, human recombinant

Catalog number

7484-10

Size

10 μg

Price

300.00 EUR

Supplier

Biovision

Order

Details

Target

Carbonic Anhydrase 4/CA4

Background

Carbonic anhydrase 4 (CA4) is also known as Carbonate dehydratase IV, Carbonic anhydrase IV, which belongs to the alpha-carbonic anhydrase family. CA4 may stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid. The enzyme regulation is activated by histamine, L-adrenaline, D-phenylalanine, L- and D-histidine. CA4 can interact with SLC4A4.

Concentration

N/A

Molecular weight

This protein is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 31 kDa. The predicted N-terminus is Ala 19. DTT-reduced Protein migrates as 33 kDa in SDS-PAGE.

Synonym

CA4, Carbonic anhydrase 4

Other name

CA4, Carbonic anhydrase 4

NCBI gene number

762

NCBI gene

CA4

Gene source

Human

NCBI number

P22748

Recombinant

Yes

Source

HEK293 cells

Purification

≥92%

Tested applications

SDS-PAGE, N/A

Tested activity

N/A

Biological activity

Measured by its esterase activity. The specific activity is >2 pmol/min/µg.

Reesults

Measured by its esterase activity. The specific activity is >2 pmol/min/µg.

Binding ability

N/A

Unit

N/A

Storage condition

-20°C

Shipping under

Gel Pack

Physical appearance

Lyophilized

Physical properties

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.

How to reconstitute

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Aa sequence

N/A

Before use

Centrifuge the vial prior to opening.

Notes

For Research Use Only! Not to be used in humans

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Additional source

Recombinants or rec. proteins

Group

recombinants