Human CellExp™ Osteoprotegerin / TNFRSF11B, human recombinant

Osteoprotegerin / TNFRSF11B

Tumor necrosis factor receptor superfamily member 11B (TNFRSF11B) is also known as Osteoclastogenesis inhibitory factor (OCIF), Osteoprotegerin (OPG). TNFRSF11B is a secreted homodimer protein, which can interact with TNFSF10 and TNFSF11. TNFRSF11B acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. TNFRSF11B inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.

N/A

This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 44.4 kDa. The predicted N-terminus is Glu 22. DTT-reduced Protein migrates as 55 kDa due to glycosylation.

TNFRSF11B, OCIF, OPG, Osteoprotegerin

TNFRSF11B, OCIF, OPG, Osteoprotegerin

4982

TNFRSF11B

Human

O00300

Yes

HEK293 cells

≥95%

SDS-PAGE, N/A

N/A

Measured by its binding ability in a functional ELISA. Immobilized rh TNFRSF11B at 10 μg/ml (100 μl/well) can bind human TNFSF11 Fc Chimera with a linear ranger of 4-256 ng/ml.

N/A

N/A

N/A

-20°C

Gel Pack

Lyophilized

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.

N/A

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Recombinants or rec. proteins

recombinants