Protein Disulfide Isomerase (PDI), human recombinant

Catalog number
7601-5000
Name
Protein Disulfide Isomerase (PDI), human recombinant
Size
5 mg
Price
4100.00 EUR
Supplier
Biovision
Order

Details

Synonyms
Protein Disulfide Isomerase
Alternates names
Protein Disulfide Isomerase, Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta, p55
Taglines
Catalyzes disulfide exchange and protein folding
Biomolecule/Target
NA
NCBI Gene ID #.
5034
NCBI Gene Symbol
PDIA1
Gene Source
Human
Accession #
P07237
Recombinant
Yes
Source
E. coli
Purity
≥95%
Activity (Specifications/test method)
Recombinant PDI is biological active. Reductase activity is 1.0 x 10e3 ∆650nm/ min-2, by measuring the turbidity increase at 650 nm due to insln reduction. The activity is expressed as the ratio of the slope of a linear part of the turbidity curve to the lag time. Isomerase activity is 0.5 µmol active RNase A min-1 µmol PDI-1, according to the re-activation of reduced and denatured RNase A.
Biological activity
NA
Results
N/A
Molecular Weight
57.1 kDa
Storage Temp.
-20°C
Shipping
gel pack
Shelf Life
1 year
Concentration
N/A
Appearance
Lyophilized protein
Physical form description
Sterile filtered and lyophilized with no additives
Reconstitution Instructions
Reconstituted PDI should be stored in working aliquots at -20°C. For long-term storage, it is recommended to add carrier protein (0.1% HAS or BSA).
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source
Recombinants or rec. proteins
Group
recombinants