BRG1 bromodomain (1448-1575 aa) (GST-tagged), human recombinant

BRG1

The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. BRG1 is a member of the SWI/SNF protein family, which forms part of a large ATP-dependent chromatin remodeling complex. This complex is required for transcriptional activation of genes normally repressed by chromatin. BRG1 is mutated in many cancer cell lines, such as breast, prostate, lung, pancreas and colon. Further, BRG1 has an important role as a tumor suppressor. This protein can be used for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.

N/A

41.8 kDa (1448-1575 aa + GST Tag)

Human recombinant BRG1 bromodomain (1448-1575 aa) (GST-tagged)

Protein BRG-1, BAF190A Mitotic growth and transcription activator, BRG1-associated factor 190A, ATP-dependent helicase SMARCA4.

6597

SMARCA4

Human

P51532

Yes

E. coli

≥95%

SDS-PAGE, N/A

N/A

N/A

N/A

N/A

N/A

N/A

-80°C

dry ice

Liquid

50 mM Tris, pH 7.5, containing 500 mM sodium chloride, 5% glycerol, and 5 mM β-mercaptoethanol.

N/A

N/A

Centrifuge the vial prior to opening.

For Research Use Only! Not to be used in humans.

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Recombinants or rec. proteins

recombinants